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. 2023 Mar 13;136(5):578–587. doi: 10.1097/CM9.0000000000002278

Figure 3.

Figure 3

Overexpression and location of hTERT protein in hVWFs. (A) Evaluation of relative hTERT mRNA expression relative to GAPDH in i-hVWFs and hVWFs by RT-qPCR analysis; (B) Evaluation of hTERT protein expression in i-hVWFs and hVWFs by WB analysis; (C) Evaluation of hTERT protein expression and location of hTERT protein by immunofluorescent staining. ∗Indicates P < 0.05. Scale bar = 100 μm and scale bar = 10 μm in the enlarged image. GFP: Green fluorescent protein; hTERT: Human telomerase reverse transcriptase; hVWFs: Human vaginal wall fibroblasts; i-hVWFs: Immortalized hVWFs; RT-qPCR: Real-time quantitative polymerase chain reaction; WB: Western blotting.