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. 2023 Mar 13;136(5):578–587. doi: 10.1097/CM9.0000000000002278

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Representative gene expression of i-hVWFs at different passages. RT-qPCR for mRNA expression and western blotting for protein expression of COL1A1 (A), COL3A1 (B), ELN (C), MMP2 (D), MMP3 (E), MMP9 (F), TIMP1 (G), and TIMP2 (H) at passage 5, 10, 20, and 30, respectively. Scale bar = 100 μm. COL1A1: Collagen type I alpha 1 chain; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; i-hVWFs: Immortalized hVWFs; MMP2: Matrix metalloproteinase 2; RT-qPCR: Real-time quantitative PCR; TIMP1: Tissue inhibitors of metalloproteinase 1.