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. 2023 Apr 3;14:1151780. doi: 10.3389/fimmu.2023.1151780

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Copyright © 2023 Park, Dean, Jiyarom, Gangcuangco, Shah, Awamura, Ching, Nerurkar, Chow, Igno, Shikuma and Devendra

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of circulating monocyte levels among the groups. Representative flow cytometry gating strategy for identification of monocytes and monocyte subsets in PBMC from NG, RG, and PG groups. Lymphocytes and monocytes were selected using CD45⁺ followed by gating for CD11b⁺ cells. (A1) non-classical (CD14^lo/CD16⁺), (A2) intermediate (CD14⁺/CD16⁺, (A3) Classical (CD14⁺/CD16^-), and (B) Total monocyte percentages as a proportion of total identified CD45⁺ cells in NG, RG, and PG groups. (C) Total monocyte counts in NG, RG, and PG groups. Mann-Whitney-U Test *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant.