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. 2023 Apr 3;13:1146477. doi: 10.3389/fonc.2023.1146477

Table 2.

New progress of hydrogel scaffolds.

Research purpose Innovation Results Ref.
Model with fibrous matrix and blood vessels The embedded gel was prepared by combining type I collagen and fibrin The model can be used to study tumor-matrix interactions in HCC (37)
Multicellular heterogeneous spheres Hanging drop method, co-culture of HCC matrix and fibroblasts, encapsulation of collagen gel, The model is much closer to ECM (38)
Novel hydrogel scaffolds HA3P50 scaffold based on hyaluronic acid and poly (methylethylene ether-Alt-maleic acid) HepG2 cells were protected from the damaging response on 2D medium (39)
New tumor microsphere model Biosynthesis of PEG-fibrinogen gels Similar in size and shape to tumors in vivo (40)
New 3D culture platform Gelatin and alginate complement each other, PEGDA incorporation controls cross-linking density A 3D alginate culture platform whose pore size can be changed artificially was made (35)
Alginate gel microarray Alginate gel micropores were prepared by electrodeposition of alginate gel on ITO electrodes. HepG2 spheres were successfully prepared (41)
Porous alginate beads Dual aqueous emulsion, controllable pore size, good biocompatibility, can directly encapsulate cells The activity, proliferation of investigated cells were increased (42)
Peptide hydrogel Max8β was used as hydrogel to form nanofibrils through hydrophobic collapse and hydrogen bonding Custom hydrogels for porosity, permeability and mechanical stability (43)
Soluble gelatin based cell carrier Temperature sensitive gelatin microspheres were mixed with alginate saline gel as cell carriers. A new platform was developed for drug testing and oncology (44)
New gel sphere Halo-linked 3D microgels of HA-MA and GelMA in air were prepared on superhydrophobic surfaces. The shape, size and cell number of the microtumor can be easily controlled. (45)
New gel matrix The basement membrane extract was gelatinized with Matrigel to form a new matrix Better mimic a single tumor in the body (46)
New alginate hybrid gel beads Acellular liver matrix and alginate constitute new hybrid gel beads HCCLM3 cells showed higher cell viability and metastatic potential (34)
Hydrogel microarray Application of optical crosslinking technology in micro machining and micro forming. Produce custom size tumor microspheres (47)
New Liquid Marble Culture Platform It forms integrates hydrogel components, replaces liquid with hydrogel and removes hydrophobic shell The liver specific function and DNA content of HM globules increased after long-term culture (48)
New hydrogel Magnetic hydrogels were prepared by combining the assembly of magnetic nanoparticles Enhances cell-cell interactions and promotes spontaneous formation of multicellular spheres (49)
New hydrogel The copolymer reversibly gelatinized in aqueous and redissolved without degrading the synthetic scaffolds A temperature responsive hydrogel was developed (50)
3D culture and drug resistance system The resistance of HepG2 cells to Bio-Pa NPs was detected in 2D and 3D cultures, respectively HepG2 cells in 3D hydrogels were more resistant to Bio-Pa NPs treatment (51)
New hydrogel Low temperature CMCH hydrogel solution gelatinizes rapidly at 37°C Hydrogels promote cell survival and proliferation, and have good biocompatibility (52)