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. 2023 Apr 3;13:1146477. doi: 10.3389/fonc.2023.1146477

Table 4.

New progress of microfluidic chips.

Research purpose Innovation Results Ref.
Cancer cell migration model 3D collagen barrier is formed through polyelectrolyte composite solidification process Form 3D aggregates of cells similar to cancer tumors, mimicking the migration of cancer cells in vivo (106)
Microarray cell culture system 1156 square microcontainers, large capacity, to ensure nutrient supply external bioreactor Preserve the function of rat primary hepatocytes for more than 2 weeks (107)
New microfluidic platform The microfluidic platform can realize the microfluidic control with many repetitions and long duration High-throughput production of tumors of uniform size; Various 3D tumor microspheres produced in the device (108)
New cell culture methods Gelatin-based 384-well ready-to-use microscaffold array It is suitable for a variety of tumor cells and can be used for drug resistance detection and tumor cell culture (109)
New manufacturing of cell sphere Construct a multicellular culture platform using acoustic fluid This produces more than 6000 tumor spheres per operation and shortens the tumor sphere formation time to one day. (110)
New microfluidic chip Polydimethylsiloxane is made of a double casting technique with a thermal aging step The tumor microsphere culture time of up to 4 weeks can observe the reaction of tumor microsphere for a long time (111)
Novel TME model Cancer cells in the microcapsule were encapsulated with a hydrogel shell to form a 3D vascularized tumor The angiogenesis in the 3D microenvironment of human tumor was simulated (112)
New 3D co-culture model Panc-1 tumor spheres were co-cultured with pancreatic stellate cells using microarray chips and The role of pancreatic stellate cells in tumor development and metastasis was studied (113)