TABLE 1.
Extracellular enzyme analyzed in our study.
| Target | Enzyme (endo or exolytic) | Substrate |
| Cellulose | endo-1,4-β-D-glucanase (endo) | Azo-CM-Cellulose |
| Cellobiohydrolase (exo) | MUF-β-D- cellobioside | |
| β-glucosidase (exo) | MUF-β-D-glucopyranoside | |
| Hemicellulose | endo-1,4-β-D-xylanase (endo) | Azo-Xylan (Birchwood) |
| β-xylosidase (exo) | MUF-β-D-xyloside | |
| Pectin | endo-1,4-β-D-mannanase (endo) | Azo-Carob Galactomannan |
| endo-1,5-α-L-arabinanase (endo) | Red Debranched Arabinan (Sugar Beet) | |
| β-galactosidase (exo) | MUF-α-D-galactoside | |
| β-mannosidase (exo) | MUF-β-D-mannoside | |
| α-glucuronidase (exo) | MUF-α-D-glucuronide hydrate | |
| Starch | α-amylase (endo) | Red Starch |
| Pullulanase (endo) | Red Pullulan | |
| Lignin | Laccase (endo) | ABTS |
| Proteins | endo-protease (endo) | Azocasein |
| Chitin | Chitinase (exo) | MUF-β-D-N.N-diacetylchitobiose hydrate |
| Phosphate monoester | Acid phosphomonoesterase (exo) | MUF-phosphate |
| Triglycerides | Lipase (exo) | MUF-oleate |
MUF, 4-Methylumbelliferone; ABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid).
All MUF based substrates and ABTS were purchased from Sigma-Aldrich, other substrates were purchased from Megazyme.