Table 2.
Primers used for quantitative PCR.
| Gene1 | Accession no. | Primer2 (5′-3′) | Length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| GAPDH | XM_027449740 | F: TGCCATCACAGCCACACAGA | 166 | 60 |
| R: ACACCCAAGGAAGGCCAACA | ||||
| β-actin | EF667345 | F: GATGAATCCGGACCCTCCAT | 71 | 60 |
| R: AAGGGTGTGGGTGTTGGTAA | ||||
| 18S rRNA | XR_003493879 | F: GAGCCTGCGGCTTAATTTGA | 121 | 60 |
| R: AACTAAGAACGGCCATGCAC | ||||
| HMBS | XM_027444343 | F: TGGACCAAATGACGATGTGC | 174 | 60 |
| R: CCACAGGTTTAGCAGGCATC | ||||
| HPRT1 | XM_027465333 | F: GCTTGAACGCTGGCAAGATA | 68 | 60 |
| R: TGCAGTCTCAGTCCTGTTGT | ||||
| TBP | XM_027454201 | F: TTAGCCCGATGATGCCGTAT | 198 | 60 |
| R: GGGCTGTGGTAAGAGTCTGT | ||||
| RPL13 | XM_027466423 | F: GCATGATCCTGAAGCCGCAC | 166 | 60 |
| R: TGGTCGGACACCTCACGATG | ||||
| YWHAZ | XM_027452537 | F: TGCTGCTGGAGATGACAAGA | 106 | 60 |
| R: TCTGATGGGATGTGTTGGCT |
1GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; β-actin, beta-actin; 18S rRNA, 18S ribosomal RNA; HMBS, hydroxymethylbilane synthase; HPRT1, hypoxanthine phosphoribosyltransferase 1; TBP, TATA box binding protein; RPL13, ribosomal protein L13; YWHAZ, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta polypeptide.
2F, forward; R reverse.