Table 5.
Average expression stability values of candidate reference genes calculated by BestKeeper.
| Gene | Standard deviation (SD) | Coefficient of variation (CV) | Correlation of coefficient (r) | p | Rank |
|---|---|---|---|---|---|
| HMBS | 0.001 | 2.76 | 0.654 | 0.001 | 1 |
| YWHAZ | 0.001 | 2.54 | 0.862 | 0.001 | 1 |
| β-actin | 0.007 | 4.82 | 0.036 | 0.898 | 2 |
| RPL13 | 0.015 | 2.68 | 0.438 | 0.090 | 3 |
| HPRT1 | 0.043 | 3.12 | 0.505 | 0.046 | 4 |
| GAPDH | 0.102 | 2.21 | 0.868 | 0.001 | 5 |
| TBP | 0.254 | 4.04 | 0.655 | 0.006 | 6 |
| 18S rRNA | 0.754 | 3.48 | 0.579 | 0.019 | 7 |
GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; β-actin, beta-actin; 18S rRNA, 18S ribosomal RNA; HMBS, hydroxymethylbilane synthase; HPRT1, hypoxanthine phosphoribosyltransferase 1; TBP, TATA box binding protein; RPL13, ribosomal protein L13; YWHAZ, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta polypeptide.