FIGURE 2.

Methodological approaches to study NVC in mice. (A) Schematic view of different experimental approaches, from slice experiments to freely moving animals with miniscopes. (B) Distinction between arteriole, covered by smooth muscle cells, and capillary, covered instead by perycites. (C) Examples of two fluorescent dyes to label blood vessels. Alexa-Fluor 633 (magenta) selectively labels arterioles while fluorescein dextran (green) labels the lumen of both arterioles and capillaries. Image adapted from Shen et al., 2012. (D) Analytical approaches to measure NVC. Top, measurement of RBCs velocity. Adapted from Chaigneau et al. (2003). Bottom, measurement of vessel diameter. Adapted from Cai et al., 2018.