TABLE 1.

Description of all primers related to Gallus gallus HSP genes and the reference genes used for RT-qPCR analysis.

Gene	Full name	Sequence (5′–3′)	Function
HSP27	Heat Shock Protein 27	F: ACA​CGA​GGA​GAA​ACA​GGA​TGA​G	Heat Shock Response
		R: ACTGGATGGCTGGCTTGG
HSP40	Heat Shock Protein 40	F: GGCATTCAACAGCATAGA	Heat Shock Response
		R: TTC​ACA​TCC​CCA​AGT​TTA​GG
HSP60	Heat Shock Protein 60	F: TTG​ATG​GAG​AAG​CCC​TCA​GC	Heat Shock Response
		R: TCC​TCT​CCA​AAC​ACA​GCA​CC
HSP70	Heat Shock Protein 70	F: AAG​GGT​AAG​CAC​AAG​CGT​GA	Heat Shock Response
		R: GCA​CGA​GTG​ATG​GAG​GTG​TA
HSP90	Heat Shock Protein 90	F: TCC​TGT​CCT​GGC​TTT​AGT​TT	Heat Shock Response
		R: AGGTGGCATCTCCTCGGT
GAPDH	Glyceraldehyde-3 Phosphate Dehydrogenase	F: AGAACATCATCCCAGCGT	Reference Gene
		R: AGC​CTT​CAC​TAC​CCT​CTT​G
HPRT1	Hypoxanthine Phospho-Ribosyl Transferase 1	F: AGC​CCC​ATC​GTC​ATA​TGC​T	Reference Gene
		R: AGC​CCC​ATC​GTC​ATA​TGC​T
BMP2	Bone Morphogenetic Protein 2	F: GCC​AGA​AAC​AAG​TGG​GAA​AA	Reference Gene
		R: TAC​GGT​GAT​GGT​AGC​TGC​TG
B-Actin	Beta-Actin	F: ACCCCAAAGCCAACAGA	Reference Gene
		R: CCA​GAG​TCC​ATC​ACA​ATA​CC
RPL5	Ribosomal Protein L5	F: AAT​ATA​ACG​CCT​GAT​GGG​ATG​G	Reference Gene
		R: CTT​GAC​TTC​TCT​CTT​GGG​TTT​CT

Abbreviations: F, forward; R, reverse.