TABLE 1.
Overview of the composition of the three immersion media used in the study.
| Constituents | Immersion media (concentration = g/L) | ||
|---|---|---|---|
| AS# | HBSS | SBF | |
| Calcium Chloride (CaCl2) | 0.13 | 0.14 | 0.292 |
| Magnesium Chloride (MgCl2.6H2O) | 0.05 | 0.1 | 0.311 |
| Magnesium Sulfate (MgSO4-7H2O) | - | 0.1 | - |
| Potassium Chloride (KCl) | 1.2 | 0.4 | 0.225 |
| Potassium Phosphate (K2HPO4.3H20) | 0.35 | 0.06 | 0.231 |
| Sodium Bicarbonate (NaHCO3) | - | 0.35 | 0.355 |
| Sodium Chloride (NaCl) | 0.85 | 137.9 | 8.035 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | - | 8 | - |
| D-Glucose (Dextrose) | - | 10 | - |
| Sodium sulphate (Na2SO4) | - | - | 0.072 |
| IM hydrochloric acid | - | - | 39–44 mL |
| Tris Buffer | - | - | 6.118 |
| Mucin | 3.5% | * | - |
| Methylparaben/Methylhydroxybenzoate* | 0.8 | - | - |
| Benzalkonium chloride | 0.02 | - | - |
| EDTA | * | - | - |
| Xylitol | * | - | - |
| Peppermint and spearmint oils | * | - | - |
*
Exact values not available # Composition derived from Reijden et al. (1996).