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. 2023 Apr 12;16:1533–1551. doi: 10.2147/JIR.S403928

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© 2023 Li et al.

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Analysis of DEGs during the development of NE-BE-EAC. (A) The limma package of R software was used to identify DEGs between NE and BE based on RNA-seq data of 94 NE tissues and 113 BE tissues from the GEO database. (B) The ClusterProfiler package in R software was used to perform GO analysis and KEGG analysis of up- and down-regulated DEGs. Similarly, DEGs between NE and EAC were analyzed based on RNA-seq data of 94 NE tissues and 147 EAC tissues in the GEO database (C), And the GO and KEGG analysis were performed using ClusterProfiler package in R software (D). Based on the RNA-seq data of 113 BE tissues and 147 EAC tissues in the GEO database, the DEGs between BE and EAC were analyzed (E), and the GO and KEGG analysis were performed using ClusterProfiler package in R software (F).