Table 2.
Dilutions of antibodies used for immunofluorescence staining
1°/2° Antibody | Description | Dilution |
---|---|---|
BRCA1 | Santa Cruz Biotech-6954 | 1:100 |
γH2AX, | Novus Biologicals 100-384 | 1:100 |
pBRCA1 | Thermofisher PA536627 | 1:100 |
YOYO-1 Iodide (491/509) | Thermofisher Y3601 | 1:5000 |
Goat anti-Mouse IgG, Alexa Fluor 568 | Thermofisher A-11004 | 1:500 |
Goat anti-Rabbit IgG, Alexa Fluor 568 | Thermofisher A-11011 | 1:500 |