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. 2023 Jan 27;108(4):629–644. doi: 10.1093/biolre/ioad009

Figure 2.

Figure 2

The transcriptome-wide temporal changes of eIVFG-derived follicles during ex vivo ovulation examined by single-follicle RNA-seq. (A) PCA of the first two principal components for follicles treated with hCG for 0, 1, 4, and 8 hours. N = 9, 13, 12, and 10 follicles at 0, 1, 4, and 8 hours, respectively. (B–D) Volcano plots of all DEGs (FDR < 0.05, fold change >2 or <0.5 compared to follicles at 0 hour). Purple: Upregulated genes; gray: non-significantly altered genes; yellow: downregulated genes. The top 10 upregulated and downregulated DEGs were highlighted in each volcano plot. (E) Comparisons of DEGs at three post-hCG time points (1, 4, and 8 hours vs. 0 hour). (F) Correlation analysis of top 66 LH-ERK1/2 target genes at 4 hours post-hCG injection in the study by Fan et al. in a mouse superovulation model and the same set of genes in the RNA-seq dataset at 4 hours post-hCG in our ex vivo ovulation model, with eight established ovulatory genes highlighted.