Figure 8.

OSIC1‐mediated stomatal closure and H₂O₂ accumulation in guard cells were dependent on PalCuAOζ. (a) Stomatal closure in the leaves of WT, palcuaoζ‐ko and palcuaoζ‐ko/OSIC1‐OE plants treated with ABA. (b) The length, width and area from at least 19 stomata using the ImageJ software. Error bars indicate SE (n ≥ 19). Asterisks indicate significant differences: *P < 0.05, **P < 0.01. The ‘ns’ represents no sense. (c) The H₂O₂ content in guard cells after ABA treatment was indicated by the H₂O₂ indicator dye H₂DCFDA. (d) The fluorescence intensity in guard cells (c) was calculated. Error bars indicate SE (n ≥ 3). Asterisks indicate significant differences: **P < 0.01.