Figure 6.

SH11 affects lignin biosynthesis via binding GH2 promoter. (a–c) Fluorescence images of longitudinal sections across flower pedicel junction stained by acridine orange in GIL292 (a), OE‐GH2‐1 (b) and OE‐GH2‐2 (c), respectively. Scale bars, 100 μm. (d) Comparison of the BTS value at 28 days after pollination in GIL292, OE‐GH2‐1 and OE‐GH2‐2. The g.f represents the gravitational unit of force. Data are presented as mean ± SD (n = 35). **P < 0.01, Student's t test. (e) The region tested for binding of SH11 in the GH2 promoter. (f) Yeast one‐hybrid analysis of OgSH11 and OsSH11 binding to GH2. The promoter sequences were fused with lacZ reporter and the blue colonies indicated interaction between SH11 and promoter. (g) EMSA revealed that both His‐OgSH11 and His‐OsSH11 were able to bind to the GH2 promoter, and His‐OgSH11 showed higher affinity.