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. 2023 Feb 28;21(5):1044–1057. doi: 10.1111/pbi.14017

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© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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TN1 interacts with TIF1. (a) Phylogenetic analysis of the TN1 protein in 14 species. Monocots and dicots are indicated in orange and green respectively. (b) TN1 recognizes the H3K27me3 peptide in pull‐down assay. (c) Yeast two‐hybrid assay showing interactions of different genotypes of TN1 with TIF1. (d) Luciferase complementation image (LCI) assay in Nicotiana Benthamian leaves. The fluorescence intensity of TN1^hap1 and TIF1 was set as 1, and the assay was repeated eight times. (e) Bimolecular fluorescence complementation (BiFC) assay in rice protoplasts. Scale bar = 10 μm. (f) TN1 directly interacts with TIF1 in Co‐IP assay. Black arrow indicates TIF1‐Myc.