Fig. 7.

STING-NLRP3-GSDMD axis inhibition suppresses hepatic ROS generation

(A-B) Wild-type mice were treated with either olive oil or CCl₄ for 6 weeks in addition to either vehicle STING inhibitor C-176 (20 mg/kg) or NLRP3 inhibitor MCC950 (20 mg/kg). Hepatic O⁻ was analyzed by DHE. (C–D)Sting^+/+ and Sting^−/− mice were injected with olive oil or CCl₄ for 6 weeks. Nlrp3^fl/fl and Nlrp3^ΔHep mice were injected with normal saline or TAA for 8 weeks. The protein level of 4HNE was analyzed by WB. (E) The levels of GSH in the liver were quantified. (F)Gsdmd^+/+ and Gsdmd^−/− mice were injected with normal saline or TAA for 8 weeks. The protein level of 4HNE was analyzed by WB. n = 6/group. *p < 0.05, **p < 0.01, ***p < 0.001.