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. 2022 Dec 19;42(8):e111500. doi: 10.15252/embj.2022111500

Figure EV1. CIN‐related mutations in bir1∆‐ad2 strains are associated with decreased aneuploidy.

Figure EV1

  1. Ten‐fold serial dilutions on the indicated media are shown. Strains engineered with the ubp6(E256X) aneuploidy tolerance mutation show no bir1∆ suppression. Strains with both the ubp6(E256X) mutation and a bir1∆ suppressor mutant do not additionally rescue the loss of CPC activity.
  2. Ten‐fold serial dilutions on the indicated media are shown. Strains have disomy of either chromosome 8 (chr8) or chromosome 10 (chr10) with or without the bir1∆ suppressor mutations. The combination of either type of disomy with any of the three suppressors shows no change in growth.
  3. Ten‐fold serial dilutions of the indicated strains were made on YPAD plates containing either 0.1% DMSO or 10 μg/ml of benomyl. Strains were adapted to persistent loss of CPC function for different amounts of time with or without BIR1 then restored. Those bir1∆‐ad strains that developed suppressor mutations after the extended growth period (bir1∆‐ad2(62F) and bir1∆‐ad2(50C)) were better able to cope with benomyl than one that did not develop suppressors (bir1∆‐ad2(58B)).
  4. Histogram showing the proportion of strains that are aneuploid for specific chromosomes in bir1∆‐ad2 strains that have identified suppressor mutations, and those that do not.