Skip to main content
. 2022 Dec 19;42(8):e111500. doi: 10.15252/embj.2022111500

Figure EV3. Suppression of bir1∆ by Mps1 mutations occurs through decreased kinase activity that does not inhibit spindle assembly checkpoint activity.

Figure EV3

  1. Ten‐fold serial dilutions on the indicated media are shown. Strains engineered with the indicated mutations were tested for rescue of BIR1 deletion. Diploid strains were heterozygous for either an identified suppressor mutation or a full deletion of the gene.
  2. Quantification of the proportion of prometaphase cells with Bub3‐mNeonGreen foci with or without 15 μg/ml nocodazole. The mps1(V631M) mutation does not affect the percentage of cells with spindle assembly checkpoint foci. Data are from a single experiment.
  3. Growth assays conducted with an ATP‐analog (1NM‐PP1) that specifically inhibits the mps1‐as1 allele. Strains with wild‐type Mps1 were not affected by the inhibitor. The WT strain is CCY1905. Data are from two independent experiments.
  4. Ten‐fold serial dilutions on the indicated media are shown. Strains engineered with the indicated mutations were tested for rescue of BIR1 deletion. Dam1 alleles with nonphosphorylatable mutations in Mps1 phosphosites (S218 and S221) do not rescue growth after bir1∆.