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. 2022 Dec 19;42(8):e111500. doi: 10.15252/embj.2022111500

Figure EV5. Engineering and characterization of FBXW7 mutant cell lines.

Figure EV5

  1. Strategy implemented to introduce specific point mutations in FBXW7 in HAP1 cells. Transfected cell lines were originally screened for a silent mutation that creates an EcoR1 restriction site.
  2. Sanger sequencing confirms the presence of the G437S mutation in FBXW7.
  3. Cell lines mutant for FBXW7 have an increased colony size after 12 days of growth in comparison to a cell line with wild‐type FBXW7. Means and standard deviations are shown. Each individual value point is the size measurement of a single clone. Data are from two independent experiments. (***) P < 0.001; (****) P < 0.0001; unpaired t‐test.