Skip to main content
. 2022 Dec 19;42(8):e111500. doi: 10.15252/embj.2022111500

Figure 6. Cdc4 limits CPC localization in prometaphase independently of Sgo1.

Figure 6

  1. Serial dilutions of strains engineered with the indicated mutations were tested for rescue of BIR1 deletion. Ten‐fold serial dilutions on the indicated media are shown. The met30‐6 and cdc4‐1 alleles are temperature‐sensitive. Two independent clones of each of these alleles are shown. The serial dilutions were performed at the permissive temperature (20°C).
  2. The amount of mNeonGreen‐Sli15 (CPC member) located at kinetochores (Nuf2‐mCherry) was measured in prometaphase. Sgo1 was depleted by placing it under a galactose‐inducible promoter and switching to glucose‐containing media (YPAD). Representative images are on the left. Scale bar is 2 μm. Averages from three independent experiments are shown.
  3. Measurements of Aurora B intensity at the inner centromere of prometaphase kinetochores. Inner centromeres were identified as the regions directly between two centromeres (ACA staining). Images from an example WT cell are shown to the right. Scale bar is 5 μm long. Each data point is an individual chromosome. More than 15 cells for each cell line were measured across two independent experiments. The mean (red line) is shown.
  4. Time line of adaptation to high levels of CIN through aneuploidy and point mutations.

Data information: (ns) nonsignifcant; (*) P < 0.05; (***) P < 0.001; (****) P < 0.0001; unpaired t‐test.