Figure EV4. Aneuploidy is non‐adaptive for histone‐humanization.

1. Schematic of the re‐yeastification process. First an already histone‐humanized strain is transformed with the plasmid encoding all four yeast core histones, then is immediately re‐humanized by counterselection of the same.
2. Ploidy analysis using flow cytometry of the re‐yeastified scc4 ^D65Y strains and the parental strain that has never had human histones.
3. Frequency of 5–FOA^R‐resistant colonies following histone humanization of the re‐yeastified strains. Kruskal–Wallis test with Dunn's multiple corrections; test against the parent scc4 ^D65Y strain show that both euploid and polyploidy strains humanize at significantly higher rates, P = 0.009 and P = 0.0034, respectively. Each dot represents a biological replicate of the histone humanization assay (n ≥ 4), the central band represents the median, the box extends from the 25^th to 75^th percentile, and the whiskers represent the minimum to maximum.