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. 2023 Jan 18;42(8):e112600. doi: 10.15252/embj.2022112600

Figure 5. DASH/Dam1c mutants rescue Aurora B kinase and SAC mutants.

Figure 5

  1. Growth assays of wild type, ipl1‐2 mutants, and ipl1‐2 kinetochore double mutants at permissive (24°C) and non‐permissive temperature (32°C). Ten‐fold serial dilutions of ~ 1.0 OD600 yeast cultures were spotted onto YPD solid media and grown at the indicated temperatures for 4 and 3 days, respectively.
  2. Flow cytometry analysis of wild type, ipl1‐2 mutants, and ipl1‐2 kinetochore double mutants at the permissive (24°C) and non‐permissive temperature (30°C). Yeast cultures were grown to logarithmic phase at 24°C and divided and grown at 24 and 30°C for 6 h before cells were collected and processed for DNA content analysis using Sytox Green stain.
  3. Growth assays of wild type, mad3∆ mutants, and mad3∆ DAD1 E50D double mutants on YPD with and without 30 μg/ml benomyl. Ten‐fold serial dilutions of ~ 1.0 OD600 yeast cultures were spotted onto the indicated solid media and grown at 30°C for 3 days.
  4. Humanization of mad3∆ mutant and double mad3∆ DAD1 E50D mutant. Each dot represents a biological replicate (n ≥ 3), the central band represents the median, the box extends from the 25th to 75th percentile, and the whiskers represent the minimum and maximum.