Figure 8. DAD1 ^E50D decreases the efficiency of sporulation and spore viability.

1. Sporulation efficiency after 5 days in sporulation medium at 25°C. Example micrographs are shown to the left for both wildtype and DAD1 ^E50D SK1 strains. Right, quantification of average sporulation efficiency from all micrographs. Two‐tailed unpaired t‐test of the mean difference in sporulation efficiency. Each dot represents the fraction of cells sporulated in a single field of view (technical replicates; n ≥ 15), the central band represents the median, the box extends from the 25^th to 75^th percentile, and the whiskers represent the minimum and maximum.
2. Spore viability assay. Individual tetrads were micromanipulated on an agar surface and spores were dissected and arrayed. Genotype was determined by PCR genotyping of the DAD1 locus. Quantification from all tetrad dissections performed, total are shown below. The one‐tailed probability of observing 51% spore viability in DAD1 ^E50D SK1 over a total of 139 tetrads is P < 0.000001, assuming spore viability of ~ 90% from wild‐type SK1.
3. Genotyping of spores isolated from tetrads with either four viable spores or two viable spores. A fraction of spores with the indicated allele (gray DAD1 ^WT and pink DAD1 ^E50D) is displayed. The one‐tailed binomial test is shown for the probability of observing the frequency of the DAD1 ^E50D allele given equal segregation (DAD1 ^WT, P = 0.46; DAD1 ^E50D, P = 0.0011). Numbers below each bar indicates the total number of spores genotyped.