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. 2023 Mar 13;42(8):e110597. doi: 10.15252/embj.2022110597

Figure 1. Activation of mtDNA stress in PolgA mut/mut cells.

Figure 1

  1. Western blot analysis of the immunoproteasome subunits LMP2, LMP7 and β‐actin (loading control) in three PolgA +/+ and three PolgA mut/mut cell lines.
  2. RT‐qPCR analysis of immunoproteasome subunits Psmb8, Psmb9, Psmb10 in three different PolgA +/+ and three PolgA mut/mut cell lines.
  3. Activity assay for the catalytic subunits of the proteasome using the pan activity‐based probe (ABP) MV151, the β1/LMP2 specific LW124, and the β5/LMP7 specific MVB127 ABPs in three PolgA +/+ and three PolgA mut/mut cell lines. Resolution of β5/LMP7 in the SDS gel is hampered as both subunits are of similar molecular weight and thus do not separate in our gels. Total protein staining was used as a loading control.

Data information: Data are shown as the mean ± SEM of three biological replicates and were analyzed with two‐tailed unpaired Student's t‐test. Asterisks indicate significance as *P < 0.05.

Source data are available online for this figure.