Figure EV4. TAP1 expression and CD8⁺ T‐cell reporter activation assay.

1. Western blot analysis of TAP1 and β‐actin (loading control) and densitometric analysis from three PolgA ^+/+ and three PolgA ^mut/mut cell lines. Densitometric analysis shows mean ± SEM of three biological replicates and is presented as fold over mean of PolgA ^+/+ set to 1.
2. CD8⁺ reporter T cell background in co‐culture with PolgA ^+/+ or PolgA ^mut/mut cells. Data are provided as normalized values to the β‐galactosidase (β‐Gal) activity of co‐cultured MEFs and CD8⁺ T reporter cells. Data show mean ± SEM of three independent biological experiments.

Data information: All data were analyzed with two‐tailed unpaired Student's t‐test. Asterisks indicate significance as **P < 0.01.