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. 2023 Mar 13;42(8):e110597. doi: 10.15252/embj.2022110597

Figure 7. mtDNA transfection activates Influenza A‐specific CD8+ T cells.

Figure 7

  1. Scheme of the immunoproteasome‐specific T cell assay detecting the HLA‐A2 restricted GILGFVFT peptide in phLF.
  2. Flow cytometric detection of Influenza‐M1/HLA‐A2 complex in phLF upon transfection with mtDNA, mtDNA pretreated with DNase I, or treatment with IFNβ (n = 3 independent experiments).
  3. Scheme of M1/HLA‐A2 restricted CD8+ T cells (4VA1 T cell clone) activated by mtDNA stressed phLF that had been loaded with Influenza‐M1 peptide.
  4. CD8+ T‐cell activation assay of Influenza‐M1/HLA‐A2 restricted CD8+ T cells upon co‐culture with mtDNA‐transfected phLFs loaded with M1 peptide or upon treatment with IFNβ or IFNγ displaying secretion of IL‐2 from three different biological experiments.

Data information: All data are shown as mean ± SEM and analyzed with one‐way ANOVA test. Asterisks indicate significance as *P < 0.05, **P < 0.01.

Source data are available online for this figure.