Figure 7. Batf regulates IL‐2–Stat5 signaling in Th17 cells under long‐term culture conditions.

• A–C
  Naïve CD4⁺CD62L^hi T cells from IL‐17a reporter mice (Il17a ^eGFP) were cultured under Th17‐polarizing conditions. On day 5, eGFP⁺/IL‐17a⁺ cells were sorted (Th17(R1)) and cultured for two additional rounds in the presence of anti‐CD3 alone (TCR(R3)) or Th17‐polarizing cytokines (Th17(R3)). Cells from round 1 (Th17(R1)) and round 3 (TCR(R3) and Th17(R3)) were assessed for surface CD25 expression (A) and intracellular pStat5 expression (B) by flow cytometry (A, B), and Ets1, Runx1, and Stat5 binding at the indicated genomic regions by ChIP–qPCR (C).

Data information: Data are mean ± s.e.m. of three independent experiments. (*P < 0.05; **P < 0.01; one‐way ANOVA followed by Tukey's test).