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. 2022 Dec 14;113(1):186–204. doi: 10.1111/tpj.16032

Figure 6.

Figure 6

A PVX‐based minimal replicon boosts further GFP5 production in P19 co‐infiltrated patches. (a) Images of Nicotiana benthamiana leaves, representative of six independent experiments, at 4 dpi with the constructs p35S::FHA:GUS‐Intron (GUS), p35S::GFP5 (GFP), p35S::P19 (P19), and p35S::PVX:Δ25KΔCP:GFP5 (PVX‐GFP), as indicated, under UV illumination. To avoid saturation effects, the OD value of each individual Agrobacterium inoculate was 0.1, compared to 0.3 in Figures 2a, 3a, and 5a.

(b) Relative GFP fluorescence units (RFU) measured from samples depicted in (a). Black bars: mean. Error bars: standard deviations. n = 6.

(c) GFP protein accumulation in the samples depicted in (a) analyzed by Western blot. Coomassie staining of total proteins (Coom.) is used as a control for equal loading. The experiment was independently repeated two times with similar results.