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Release study of the caged fluorescent TCO derivatives 21 and 22 by the click and release reaction with tetrazine 19 or 20. A) The used tetrazines in this study. B) Study conditions, either 21 or 22 was incubated with tetrazine 19 or 20 and the fluorescence (excitation: 380 nm, emission: 450 nm) was measured at certain time points after the addition of the tetrazine. C) The release efficiency plotted as a function over time.
