Table 2.

EC₅₀ values for tetrazine 20, TCO‐DOX (27), TCO‐DOX (27)+10 equiv. 20 and doxorubicin from proliferation assays in HeLa cells determined using a CCK‐8 assay.

Compound	EC50	95 % CI [a]
Tetrazine 20	29.81 μM	24.60–36.40 μM
TCO‐DOX 27	1.654 μM	1.236–2.217 μM
TCO‐DOX 27+10 equiv 20	0.076 μM	0.064–0.096 μM
Doxorubicin	0.0045 μM	0.0031–0.0036 μM

[a] EC₅₀ 95 % confidence interval (n=6).