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. 2022 Dec 3;237(3):999–1013. doi: 10.1111/nph.18581

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© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Fig. 2 — Prunasin accumulation in Eucalyptus yarraensis and Eucalyptus camphora, and CYPs involved in prunasin production. (a) Mean prunasin content (± 1 SE) in E. yarraensis and E. camphora seedlings at 1, 4, 7, and 10 months of age (n = 8). (b) Transcript profiles of prunasin biosynthetic CYP genes in representative E. yarraensis and E. camphora individual tissue at different ontogenetic stages. Prunasin and transcript values at 1 month represent a pooled sample due to small plant size. (c) Extracted ion chromatograms (EIC) corresponding to prunasin (m/z 340.1032 [M + FA]⁻) of leaf extracts from Nicotiana benthamiana plants transiently expressing the three candidate CYP genes from E. yarraensis and E. camphora with the UGT85A59 from Eucalyptus cladocalyx. DW, dry weight; TPM, transcripts per million.