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. 2022 Dec 3;237(3):999–1013. doi: 10.1111/nph.18581

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© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Fig. 4 — Transient expression of homologous prunasin biosynthetic genes from acyanogenic Eucalyptus grandis. (a) Extracted ion chromatograms (EIC) of Nicotiana benthamiana leaf extracts prepared from plants transiently co‐expressing homologs of prunasin biosynthetic genes from E. grandis (marked in bold) with the prunasin biosynthetic genes from Eucalyptus yarraensis. The chromatograms surrounded by a gray box for the combinations co‐expressing EgCYP79A36 and EgCYP79A37, respectively, are enlarged in panel B. (b) Zoom on two chromatograms from panel A where the CYP79s EgCYP79A36 and EgCYP79A37 are each co‐expressed with downstream prunasin biosynthetic genes.