Table 1.
Lag time (t lag), elongation rate (k), remaining monomer concentration determined by SEC and maximum fluorescence after performing ThT‐based kinetic assays.
|
Solvent |
Salt concentration |
t lag [hours] |
k [h−1] |
Remaining Monomer [μM] |
% max fluorescence at 160 hours |
|---|---|---|---|---|---|
|
H2O |
NaCl 150 mM |
34.3±4.9 |
0.84±0.01 |
12.8±9.0 |
30.3±14.9 |
|
NaCl 1.5 M |
31.0±5.1 |
7.7±5.9 |
0 |
72.6±11.5 |
|
|
CsI 150 mM |
nd[a] |
0.06±0.05 |
38.1±7.8 |
7.9±6.5 |
|
|
CsI 1.5 M |
nd[a] |
0.01±0.01 |
35.9±16.6 |
2.1±1.0 |
|
|
D2O |
NaCl 150 mM |
23.8±4.0 |
2.5±0.6 |
2.0±3.0 |
98.1±0.9 |
|
NaCl 1.5 M |
19.0±2.6 |
6.1±2.5 |
0 |
69.0±15.3 |
|
|
CsI 150 mM |
39.3±0.9 |
0.65±0.36 |
11.7±2.7 |
59.1±25.1 |
|
|
CsI 1.5 M |
nd[a] |
0.32±0.23 |
0 |
12.8±8.2 |
[a] nd not determined due to lack of detectable ThT fluorescence signal.