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Saturation binding experiments of probes (A) 14, (B) 15, (C) 16, and (D) 17 in CHO-K1-hGPR35-NLUC cells. Nonspecific bindings were measured by pretreatment with 10 μM zaprinast. The data represent mean ± SD from three independent measurements, each with three replicates (n = 3).
