Enriched environment (EE) mitigates depression-like behaviors in
mice after ICH. (a and b) Mice in the ICH + EE group exhibited
less immobility time in the forced swim test (FST, a) and the
tail suspension test (TST, b) on day 28 than ICH mice housed in
a standard environment (SE). n = 8 mice/group. F = 7.47, 5.28,
respectively; *p < 0.05 vs. sham group;
#p < 0.05 vs. ICH + SE
group (one-way ANOVA followed by Bonferroni’s post hoc test).
(c) Mice in the ICH + EE group consumed more sucrose-sweetened
water in the sucrose preference test than ICH mice in the
standard environment on day 28. n = 10 mice/group. F = 21.85,
*p < 0.05 vs. sham group;
#p < 0.05 vs. ICH + SE
group (one-way ANOVA followed by Bonferroni’s post hoc test). (d
and e) In the light/dark transition test, the mice in the
ICH + EE group spent more time in the light box (d) and less
time in the dark box (e) than those in the ICH + SE group.
n = 7–8. F = 8.954, 8.954, *p < 0.05 vs.
sham group; ##p < 0.01 vs.
ICH + SE group (one-way ANOVA followed by Bonferroni’s post hoc
test) and (f) The number of entries in the lightbox was not
statistically different between the ICH + SE and ICH + EE
groups. n = 8 mice/group. F = 0.09439, Values are mean ± SD.