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. 2023 Jan 23;3(1):e654. doi: 10.1002/cpz1.654

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© 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Preliminary tests for segmentation. (A) Original. (B) Segmentation methods tested on images without PSF deconvolution and with (upper panel) or without pre‐processing (lower panel). (C) Segmentation methods tested on images with PSF Richardson‐Lucy (RL) (1 iteration) deconvolution and with (upper panel) or without pre‐processing (lower panel; MF – Median filter, RB – Rolling Ball algorithm). Examples : Cyan box: Under‐segmentation, magenta box: over‐segmentation, green box: Suitable segmentation for further fine‐tuning.