Figure 7.

Visualization of the binding of 18a and 24 at 50 nM on HEK293G cells stably expressing SNAP-CXCR4, scale bar = 20 μm. (a–d) Snap-Surface Alexa Fluor 488 labelling of SNAP-CXCR4. (e,f) 50 nM 18a and (g,h) 50 nM 24 binding in the (e,g) absence or (f,h) presence of 10 μM AMD3100 (30 min pre-incubation). Single equatorial confocal images were obtained after 30 min incubation at 37 °C in the continued presence of the fluorescent ligand and unlabeled antagonist if present. Images shown are from a single experiment representative of at least three performed.