Table 1. Binding Affinities of the Novel Fluorescent Ligands Determined in Membranes from HEK293G Cells Stably Expressing NLuc-CXCR4.
| example | pKD (log M)a | n |
|---|---|---|
| 10 (BY630/650-X) | 7.01 ± 0.11 | 4 |
| 11 (sulfo-cyanine5) | 6.46 ± 0.05 | 5 |
| 18a (BY630/650-X) | 6.82 ± 0.09 | 3 |
| 18b (BY-FL-X) | 6.62 ± 0.08 | 4 |
| 24 (BY630/650-X) | 7.07 ± 0.03 | 4 |
a
pKD value was calculated from the negative logarithm of the equilibrium dissociation constant (KD) determined from saturation-binding experiments using increasing concentrations of labeled ligand in the absence or presence of AMD3100 (10 μM). Data are expressed as mean ± SEM of n experiments, where each experiment was performed in triplicate.