Table 2. Binding Affinities (pKi) of CXCL12, AMD3100, and IT1t as Calculated from Cheng–Prusoff Analysis of the Data Displayed in Figure 4.
| IT1t |
AMD3100 |
CXCL12 |
|||||
|---|---|---|---|---|---|---|---|
| example | pIC50 | pKi | pIC50 | pKi | % max. inhibition of AMD3100a | pIC50 | pKi |
| 10 | 7.70 ± 0.13 (4) | 8.04 ± 0.11 (4) | 6.47 ± 0.20 (4) | N.D | 75.26 ± 7.08 | N.D | N.D |
| 11 | 8.18 ± 0.12 (4) | 8.50 ± 0.07 (4) | 7.16 ± 0.11 (4) | 7.45 ± 0.07 (4) | 95.20 ± 9.60 | N.D | N.D |
| 18b | 7.57 ± 0.36 (3) | 8.59 ± 0.06 (3) | 6.82 ± 0.66 (3) | 7.29 ± 0.06 (3) | 96.25 ± 3.82 | N.D | N.D |
| CXCL12AF-647 | 8.04 ± 0.01 (5) | 7.00 ± 0.04 (5) | 7.97 ± 0.07 (5) | ||||
a
Maximum inhibition of AMD3100 in respect to IT1t, calculated following baseline removal of BRET ratios measured at 10–11 M. Data were then normalized as a percentage of maximum inhibition seen at 10–5 M IT1t (100%). All values are expressed as mean ± S.E.M. Number of experiments are shown in parenthesis.