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. 2023 Mar 22;66(7):5041–5060. doi: 10.1021/acs.jmedchem.2c02132

Figure 4.

Figure 4

Biophysical characterization of compound 26e (OICR-8268) with the DCAF1 WDR domain. Binding of compound 26e to the DCAF1 WDR domain was tested by SPR and DSF assays. (A) SPR showed 26e binding to DCAF1 potently with a KD of 38 ± 1.5 nM, and (B) DSF showed that the compound stabilizes DCAF1 in a concentration-dependent manner (C) with increases in the protein’s melting temperature, indicating binding. (D) Orthogonal binding confirmation of 26e by ITC with a KD of 216 ± 76 nM. All experiments were performed in triplicate.