Fig. 2.

Effect of statins and L-OHP on Ras localization, Akt, ERK1/2, JNK, and NF-κB activation. a–c Cells were administered with statins or L-OHP. a LoVo cells were treated with statins or L-OHP alone for 3 days. b, c LoVo and Colon-26 cells were co-treated with simvastatin and L-OHP for 3 days. Protein detected by western blot assay and the amount of detected proteins were measured based on densitometry. The results are exemplary four independent experiments. Mean values and S.D. are shown. Statistical analysis was performed by ANOVA with Dunnett’s, and the difference was considered significant when P < 0.05