Table 3.
Vitamin content of five batches of the NF
| Parameter | Unit | Batch number | Method of analysis | ||||
|---|---|---|---|---|---|---|---|
| #1 | #2 | #3 | #4 | #5 | |||
| Thiamin | mg/100 g | 0.01 | < 0.05 | 0.24 | < 0.05 | 0.15 | HPLC–MS/MS; in‐house |
| Riboflavin | mg/100 g | 0.97 | 0.69 | 0.75 | 0.97 | 1.2 | HPLC‐MS/MS; in‐house |
| Nicotinamide + Nicotinic acid | mg/100 g | 1.8 | 1.7 | 1.0 | 9.0 | 9.0 | HPLC‐MS/MS; in‐house |
| Vitamin B6 (Pyridoxamine + Pyridoxal + Pyridoxine) | mg/100 g | 0.48 | 0.48 | 0.39 | 0.40 | 0.47 | HPLC‐MS/MS; in‐house |
| Cobalamin | μg/100 g | 6.62 | 3.14 | 5.88 | 7.33 | 17.7 | HPLC‐UV; AOAC: 1990 vol II 952.20 Cobalamin pag. 1082–1083 |
| Ascorbic acid | mg/100 g | 0.4 | 0.4 | < 0.2 | 1.1 | 0.7 | HPLC‐FLU; NEN‐EN 14130 |
| Vitamin D3 | μg/100 g | < 2.0 | 2.6 | 7.0 | 15.8 | 33.7 | HPLC‐MS/MS; mod. ISO 14892:2002 |
| Vitamin E (α‐, β‐, γ‐, δ‐tocopherol) | mg/100 g | 33.5 | 21.8 | 13.9 | 20.3 | 11.1 | HPLC‐FLU; NEN‐EN 12822 |
| Biotin | μg/100 g | 13.0 | 13.4 | 11.7 | 13.5 | 13.2 | HPLC‐MS/MS; in‐house |
| Phylloquinone | mg/100 g | 14.5 | 14.0 | 11.7 | 12.2 | 11.4 | HPLC‐MS/MS; in‐house |
HPLC–MS/MS: high‐performance liquid chromatography–tandem mass spectrometry; HPLC‐UV: high‐performance liquid chromatography‐ultraviolet; HPLC‐FLU: high‐performance liquid chromatography‐fluorescence detection; NEN: Nederlandse Norm; EN: Europese Norm; ISO: International Organization for Standardization; AOAC: Association of Official Analytical Collaboration.