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. 2023 Apr 17;21(4):e07903. doi: 10.2903/j.efsa.2023.7903

Table 6.

Specifications of the NF

Description: The NF is a protein concentrate produced from a mixture of Lemna gibba and Lemna minor. The manufacturing process involves protein separation from insoluble fibres, and then precipitation of this protein fraction.

Appearance: green powder

Source: Lemna gibba (70–100%) and Lemna minor (0–30%)
Parameter Specifications
Moisture 1.5–8%
Protein (N × 6.25) 60–75%
Ash 4–12%
Fat 2–11%
Fibre 6–17%
Carotenoids
β‐Carotene < 755 mg/kg
Vitamins
Phylloquinone < 16 mg/100 g
Minerals
Boron < 10 mg/kg
Copper < 12 mg/kg
Molybdenum < 40 mg/kg
Iron < 670 mg/kg
Zinc < 50 mg/kg
Manganese < 100 mg/kg
Heavy metals
Arsenic < 0.2 mg/kg
Lead < 0.3 mg/kg
Cadmium < 0.2 mg/kg
Mercury < 0.1 mg/kg
Antinutritional factors
Oxalic acid < 1,900 mg/kg
Contaminants
Lysino‐alanine < 500 mg/kg bound LAL + < 10 mg/kg free LAL
Cyanotoxins (Microcystins‐/Nodularin) < 0.19 mg/kg
Nitrate < 3,000 mg/kg
Microbiological
Total colony count < 104 CFU/g
Bacillus cereus < 100 CFU/g
Clostridium perfringens < 100 CFU/g

Coagulase‐positive

Staphylococci

< 100 CFU/g
Escherichia coli < 10 CFU/g
Enterobacteriaceae < 10 CFU/g
Listeria monocytogenes Not detected in 25 g
Salmonella spp. Not detected in 25 g
Yeasts and moulds < 10 CFU/g

LAL: Lysino‐alanine; CFU: colony forming units.