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. 2023 Jan 6;150(1):dev201373. doi: 10.1242/dev.201373

Fig. 2.

Fig. 2.

Localized expression of dominant-negative Ets1 (dnLv-Ets1.GFP) in PMCs disrupts skeletogenesis in transgenic sea urchin embryos. (A) Schematic of the transactivator and responder constructs used to induce PMC-specific GFP or dnLv-Ets1-GFP expression. (B) Experimental design showing the treatment schedules (a-d). Solid circles indicate the stages at which Dox was added. Black arrowheads indicate the stages at which embryos were collected for analysis after overnight Dox treatment. (C) Representative images of transgenic embryos with induced expression of PMC-specific GFP or dnLv-Ets1-GFP (treatment schedules a-d). GFP expression in the PMCs did not affect embryonic skeletogenesis. Induced asymmetric expression of dnLv-Ets1-GFP in PMCs inhibited spicule formation and elongation of skeletal rods (asterisks). The percentage of transgenic embryos showing similar patterns of GFP or dnLv-Ets1-GFP expression and phenotype is shown. White arrowheads indicate ventral transverse rods that developed normally. Top: GFP fluorescence in live embryos. Middle: GFP fluorescence overlaid onto DIC images. Bottom: polarized light images showing skeletal elements. Scale bars: 50 μm.