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. 2023 Mar 9;150(5):dev201231. doi: 10.1242/dev.201231

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — EGFR-dependent ERK activity is elevated in the region of epithelial morphogenesis. (A) Procedure of perivitelline space labeling. (B,C) The imaging of the perivitelline space (B) and ERK-FRET activity (C) in a stage 11 embryo (n=5). (D-F) Simultaneous live-imaging of the perivitelline space and ERK-FERT activity (Movie 1). Time (t)=0 was set at the time of tracheal invagination of T1 segment. Times are in minutes. tp, tracheal pit; seg, segmental groove.