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. 2023 Mar 9;150(5):dev201231. doi: 10.1242/dev.201231

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Apical cell extrusion at the site of vitelline membrane detachment. (A) Schematic of the ventral view of stage 11 embryos. (B-E) Active caspase staining (cDcp1) of control (B) and EGFR mutant (C) embryos. Parts of the head and thoracic regions of C (dotted box) are enlarged in D and E. Unfilled arrowheads, basally extruded cells; filled arrowheads, apically extruded cells; orange arrows, salivary gland invagination. (F,G) Time-lapse series of the ventral head and thorax imaged from two separate embryos, showing apical cell extrusion in the invaginating salivary gland primordia (orange arrows and white arrowheads). Other parts maintained tight vitelline membrane contact at this stage. Times are in minutes.