| 10 mL Serological pipette |
Fisher Scientific |
13-678-26D |
|
| 1-thio-glycerol |
Sigma |
M6145 |
|
| 2 mL Serological pipette |
Fisher Scientific |
13-678-26B |
|
| 250 mL Filter Unit, 0.2 μm aPES, 50 mm Dia |
Fisher Scientific |
FB12566502 |
|
| 35 mm Easy Grip Tissue Cluture Dish |
Falcon |
353001 |
|
| 4D Nucleofector core unit |
Lonza |
276885 |
Nucleofector |
| 5 mL Serological pipette |
Fisher Scientific |
13-678-25D |
|
| 60 mm Easy Grip Tissue Culture Dish |
Falcon |
353004 |
|
| 6-well ultra-low attachment plates |
Corning |
3471 |
|
| 9” Disposable Pasteur Pipets |
Fisher Scientific |
13-678-20D |
|
| Apo-transferrin |
Sigma |
T1147 |
|
| Bovine serum albumin (BSA) |
Sigma |
A9418 |
|
| Cell culture grade water |
Cytiva |
SH30529.02 |
|
| Chemically defined lipid concentrate |
Gibco |
11905031 |
|
| Chroman 1 |
Cayman |
34681 |
|
| Class II, Type A2, Biological safety Cabinet |
NuAire, Inc. |
NU-540-600 |
Hood, UV light |
| Costar 24-well plate, TC treated |
Corning |
3526 |
|
| Costar 6-well plate, TC treated |
Corning |
3516 |
|
| DAPI solution |
Thermo Scientific |
62248 |
|
| DMEM |
Gibco |
11965092 |
|
| DMEM/F12 |
Gibco |
11320033 |
|
| DMSO (Dimethly sulfoxide) |
Sigma |
D2438 |
|
| DPBS+/+ |
Gibco |
14040133 |
|
| Emricasan |
Cayman |
22204 |
|
| Epi5 episomal iPSC reprogramming kit |
Life Technologies |
A15960 |
|
| Essential 8-Flex |
Gibco |
A2858501 |
PSC medium with heat-stable FGF2 |
| EVOS XL Core Imaging system |
Life Technologies |
AMEX1000 |
|
| Fetal bovine serum - Premium Select |
Atlanta Biologicals |
S11150 |
|
| FGF2 |
Peprotech |
100-18B |
|
| GlutaMAX supplement |
Gibco |
35050061 |
L-alanine-L-glutamine supplement |
| Ham’s F12 Nutrient Mix |
Gibco |
11765054 |
|
| HERAcell VIOS 160i CO2 incubator |
Thermo Scientific |
50144906 |
|
| Human Anti-EN2, mouse |
Santa Cruz Biotechnology |
sc-293311 |
|
| Human anti-Ki67/MKI67, rabbit |
R&D Systems |
MAB7617 |
|
| Human anti-PTF1a, rabbit |
Novus Biologicals |
NBP2-98726 |
|
| Human anti-TUBB3, mouse |
Biolegend |
801213 |
|
| IMDM |
Gibco |
12440053 |
|
| Insulin |
Gibco |
12585 |
|
| Laminin Mouse Protein |
Gibco |
23017015 |
|
| Matrigel Matrix |
Corning |
354234 |
Basement membrane matrix |
| MEM-NEAA |
Gibco |
11140050 |
|
| Mini Centrifuge |
Labnet International |
C1310 |
Benchtop mini centrifuge |
| Monarch RNA Cleanup Kit (50 μg) |
New England BioLabs |
T2040 |
Silica spin columns |
| Monarch Total RNA Miniprep Kit |
New England BioLabs |
T2010 |
Silica spin columns |
| N-2 supplement |
Gibco |
17502-048 |
|
| Neurobasal medium |
Gibco |
21103049 |
|
| PBS, pH 7.4 |
Gibco |
10010023 |
|
| PFA 16% |
Electron Microscopy Sciences |
15710 |
|
| Polyamine supplement |
Sigma |
P8483 |
|
| Poly-L-Ornithine (PLO) |
Sigma |
3655 |
|
| Potassium chloride |
Sigma |
746436 |
|
| SB431542 |
Sigma |
54317 |
|
| See through self-sealable pouches |
Steriking |
SS-T2 (90×250) |
Autoclave pouches |
| Sodium citrate dihydrate |
Fisher Scientific |
S279-500 |
|
| Syringe filters, sterile, PES 0.22 μm, 30 mm Dia |
Research Products International |
256131 |
|
| Trans-ISRIB |
Cayman |
16258 |
|
| TRIzol Reagent |
Invitrogen |
15596018 |
Phenol and guanidine isothiocyanate |
| TrypLE Express Enzyme (1×) |
Gibco |
12604039 |
Cell dissociation reagent |
| Vapor pressure osmometer |
Wescor, Inc. |
Model 5520 |
Osmometer |
| Y-27632 |
Biogems |
1293823 |
|
