(A) Schematic showing overall study design.
(B and C) UMAP plot showing the major cellular clusters (B) by patient and (C) by cell type.
(D) Scaled expression level of cell-type-defining markers and percentage of positive cells in each cluster.
(E) UMAP plots overlaying cluster-specific expression patterns of representative cell-type-defining markers.
(F) Frequency of cells in each cluster, by patient.
(G) UMAP plots for 2 specimens before and after cryopreservation.
(H) Violin plots of representative marker expression in 2 specimens, before and after cryopreservation. UMAP, uniform manifold approximation and projection.